Mechanical characterization of regenerating Hydra tissue spheres - Service Instrumentation Conception Caractérisation
Article Dans Une Revue Biophysical Journal Année : 2024

Mechanical characterization of regenerating Hydra tissue spheres

Résumé

Hydra vulgaris, long known for its remarkable regenerative capabilities, is also a long-standing source of inspiration for models of spontaneous patterning. Recently it became clear that early patterning during Hydra regeneration is an integrated mechanochemical process whereby morphogen dynamics is influenced by tissue mechanics. One roadblock to understanding Hydra self-organization is our lack of knowledge about the mechanical properties of these organisms. In this study, we combined microfluidic developments to perform parallelized microaspiration rheological experiments and numerical simulations to characterize these mechanical properties. We found three different behaviors depending on the applied stresses: an elastic response, a viscoelastic response, and tissue rupture. Using models of deformable shells, we quantify their Young’s modulus, shear viscosity, and the critical stresses required to switch between behaviors. Based on these experimental results, we propose a description of the tissue mechanics during normal regeneration. Our results provide a first step toward the development of original mechanochemical models of patterning grounded in quantitative experimental data
Fichier principal
Vignette du fichier
BJ_resubmission_bioarxiv.pdf (946.16 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-04668523 , version 1 (07-08-2024)

Identifiants

Citer

Thomas Perros, Anaïs Biquet-Bisquert, Zacchari Ben Meriem, Morgan Delarue, Pierre Joseph, et al.. Mechanical characterization of regenerating Hydra tissue spheres. Biophysical Journal, 2024, 123 (13), pp.1792-1803. ⟨10.1016/j.bpj.2024.05.022⟩. ⟨hal-04668523⟩
154 Consultations
32 Téléchargements

Altmetric

Partager

More